Introduction: This study was conducted to assess healthcare providers’ knowledge of infection control measures in healthcare facilities in the city of Kisangani.
Materials and Methods: A descriptive cross-sectional study was conducted among 240 healthcare providers from 20 healthcare facilities in the city of Kisangani between August 15 and October 22, 2025. Data were presented as proportions for categorical variables and as mean ± SD for normally distributed quantitative variables.
Results: 67.5% of participants had completed on-the-job training on infection control measures. Knowledge of infection control measures was very high for all measures (≥80%); however, it was average for the critical moments of hand hygiene as defined by the WHO (around 60%).  Knowledge of the components of the respiratory protocol, types of gloves, masks, and routes of transmission was high (≥70%), and the overall level of knowledge was rated as good for the majority (73.7%).
Conclusion: Overall, awareness of preventive measures is good, although gaps were observed in knowledge of the critical moments for hand hygiene as defined by the WHO. This may negatively impact compliance in healthcare settings. Capacity building is needed to improve the level of knowledge